Figure 2.

Cells were treated with glutamate, adenosine, or high K⁺ (in the presence of ARL67156). However, all experiments were carried out in either siRNA(+) or siRNA(−) cells. ATP release under control conditions was unaffected by siRNA treatment, but the response to glutamate (A), adenosine (B), and 45 mM K⁺ (C) was abolished. Results are the averages of RLU values from three to four individual cultures. SEM values are indicated by vertical bars. *Statistically significant (p < .05) difference from all other groups, but not each other.

Note. RLU = relative light units; ATP = adenosine triphosphate; ENT3 = equilibrative nucleoside transporter 3; siRNA = small interfering RNA.