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. 2014 Jul 22;6(4):1759091414543439. doi: 10.1177/1759091414543439

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© The Author(s) 2014

This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 3. — mRNA expression of SLC17A9 in astrocyte cultures at the age of 1 week, 2 weeks, 3 weeks without dBcAMP, or 3 weeks with 0.25 mM dBcAMP for the last week. The figure indicates a sharp decrease in SLC17A9 expression with the maturation of astrocytes. The size of PCR product of SLC17A9 is 189 bp and TBP 236 bp. mRNA expression was quantitated by RT-PCR as ratios between SLC17A9 and TBP from five individual cultures. SEM values are indicated by vertical bars. *Statistically significant (p < .05) difference from 1-week group, and **statistically significant (p < .05) difference from all other groups.

Note. SLC17A9 = a neuronal vesicular transporter of ATP; dBcAMP = dibutyryl cyclic adenosine monophosphate; TBP = TATA box-binding protein; siRNA = small interfering RNA.