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. 2014 Sep 5;6(5):1759091414548916. doi: 10.1177/1759091414548916

Figure 5.

Figure 5.

Localization of RhoU and Krt23 in cultured Schwann cells and in sciatic nerves. (a) In primary mouse Schwann cell cultures, immunofluorescent signal for RhoU was detected within the cytoplasm (a, arrows), in membrane protrusions (a, arrowheads), and in Schwann cell processes (A, open arrowhead). (b, c) In sciatic nerves of 3-month-old mice, RhoU was expressed in myelinating Schwann cells (B, C, arrows), whereas nonmyelinating Schwann cells in Mbp-negative area did not express RhoU (c, encircled area). (d) For Krt23, a punctated staining pattern was present in the cytoplasm of cultured Schwann cells (d, arrows). Its localization was further detected in membrane protrusions (d, arrowhead) as well as along Schwann cell processes (d, open arrowhead). (e, f) Immunofluorescent microscopy on sciatic nerves of 3-month-old mice revealed Krt23 to be punctately localized in myelinating Schwann cells (e, f, arrows), as well as in nonmyelinating Schwann cells (f, encircled area). Localization in nonmyelinating Schwann cells was visualized on sciatic nerves derived from MAL-overexpressing mice (c, f). Blue: DAPI. Bars: 20 µm.