Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 26;6(5):1759091414551955. doi: 10.1177/1759091414551955

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014

This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm).

PMC Copyright notice

Figure 5. — Proliferation of oligodendrocyte progenitors cells (OPCs) in the corpus callosum following cuprizone/vehicle (Cup) or cuprizone/rapamycin (CupR). Proliferating OPCs were labeled with Ki67 (red), a marker of cell proliferation, and PDGFRα (green), a marker of OPCs. Proliferating OPCs were identified as cells that colabeled for both Ki67 and PDGFRα. (A) White arrows indicate PDGFRα+/Ki67+ cells. Upper panel: representative areas of the corpus callosum from rapamycin-treated mice (Control, Rapa, a), compared with mice treated for 4 or 6 weeks with cuprizone plus rapamycin (CupR, b, c) or allowed to recover for 1 or 7 weeks (d, e). Lower panel: representative areas of the corpus callosum from vehicle-treated tissue (Control, Veh, f) and from mice treated for 4 or 6 weeks with cuprizone (Cup, g, h) or allowed to recover for 1 or 7 weeks (i, j). Seven weeks following termination of treatment, a few PDGFRα/Ki67+ cells were still present in CupR and Cup tissue (red arrows, e, j). Images were acquired using a 40 × oil Plan Neofluor (NA 1.3) objective. Scale bar = 50 µm. (B) Quantification of proliferating OPCs at 4 and 6 weeks of treatment and 1, 3, 5, and 7 weeks of recovery. Ki67+/PDGFRα+ cells were counted from images of the right and left half of the corpus callosum, which included the midline of the cingulum to the midline of the corpus callosum with n = 3 animals per group. Values are the mean ± SD analyzed by one-way ANOVA. **p < .01, ***p < .001, ****p < .0001, with Bonferonni’s correction for multiple comparisons. Rapa = rapamycin alone; CupR = cuprizone plus rapamycin; Veh = vehicle alone; Cup = cuprizone alone; PDGFRα = platelet derived growth factorα.