Fig. 3.

Differential fate of endocytosed protein following specific knockdown of c-Yes and c-Src vs. nontargeting control at the Sertoli cell BTB in vitro. A: following knockdown of c-Yes or c-Src by ∼60 or ∼70%, respectively, the kinetics of disappearance of endocytosed/biotinylated protein from Sertoli cell cytosol was estimated. Knockdown of c-Yes delayed the disappearance of endocytosed/biotinylated JAM-A and CAR, which was analogous to a knockdown of c-Src that also delayed the disappearance of CAR from the cell cytosol. This could be the result of changes in recycling. B: knockdown of c-Yes delayed the recycling of endocytosed/biotinylated JAM-A and CAR back to the Sertoli cell plasma membrane. The data for the silencing of c-Src were not shown since consistent data were not obtained in multiple experiments. These findings illustrate that c-Yes is involved in protein recycling. In both A and B, β-actin served as protein loading control. Immunoblot data shown here were representative findings of an experiment that was repeated 4 times and yielded similar results. Each data point in the line graph is the mean ± SD of n = 4 experiments. *P < 0.05; **P < 0.01.