Fig. 5.

TG2 expression is blocked by HIF-1α inhibitor, PX-478. A: representative Western blots demonstrating the effect of cobalt chloride (CoCl₂; 300 μM) and vehicle on HIF-1α and TG2 expression under normoxia (21% O₂) or hypoxia (3% O₂) conditions at 24 h. B: bar graph demonstrating the effect of CoCl₂ and hypoxia on HIF-1α and TG2 expression measured by densitometry analysis (n = 4 blots/treatment group). C: representative Western blots demonstrating the effect of vehicle and PX-478 in presence of CoCl₂ for 24 h. Bar graph demonstrating the effect of PX-478 on CoCl₂-induced expression of HIF-1α (D) and TG2 (E) measured by densitometry analysis (n = 4 blots/treatment group). F: representative Western blots demonstrating the effect of vehicle or PX-478 under hypoxia for 24 h. G: bar graph demonstrating the effect of PX-478 on hypoxia-induced TG2 expression measured by densitometry analysis (n = 4 blots/treatment group). HIF-1α (120 kDa) was detected by immunoblotting with anti-HIF-1α antibody. TG2 protein expression (78 kDa) was detected with anti-TG2 antibody. Smooth muscle α-actin (42 kDa) was blotted on the stripped membrane as loading control. *P < 0.05, significantly different from vehicle-treated normoxia control. #P < 0.05, significantly different from CoCl₂-treated control or vehicle-treated hypoxia control.