Fig. 6.
Urine pH standardization. A: urine pH was similar between base-supplemented Car2+/+ and Car2−/− mice at 6.16 ± 0.611 and 6.36 ± 0.34, respectively (P = 0.402). B and C: 24-h bladder (B) and kidney (C) bacterial burdens were higher in Car2−/− mice than in base-supplemented Car2+/+ mice. The bladder bacterial burden was 2.39 × 106 ± 4.12 × 106 in base-supplemented Car2+/+ mice and 2.49 × 107 ± 1.47 × 107 in Car2−/− mice (P = 0.001), and the kidney bacterial burden was 24,945 ± 99,862 in base-supplemented Car2+/+ mice and 366,858 ± 665,773 in Car2−/− mice (P = 0.013). D: kidney clearance of bacteria occurred in 2 of 25 (8.0%) of Car2−/− kidneys and 7 of 28 (25%) of base-supplemented Car2+/+ kidneys (P = 0.148).