Table 1.
Antibodies used in the present study
| Antibody (Species) | Company | Catalog Number | Dilution |
|---|---|---|---|
| Primary antibodies | |||
| Aquaporin 2 (rabbit, anti-human, ATTO 550 fluorescent label) | Alomone Labs (Jerusulem, Israel) | AQP-002-AO | 1:200 |
| CA-II (rabbit, anti-human) | Epitomics (Burlingame, CA) | S2774 | 1:1,000 |
| Lcn2/Ngal (goat, anti-mouse) | R&D Systems (Minneapolis, MN) | AF1857 | 1:50 |
| Ly6G (rabbit, anti-mouse) | Biolegend (San Diego, CA) | 127607 | 1:50 |
| V-ATPase (goat, anti-human) | Santa Cruz Biotechnology (Santa Cruz, CA) | 21209 | 1:50 |
| Secondary antibodies | |||
| Alexa fluor 488 (donkey, anti-goat) | Jackson ImmunoResearch (West Grove, PA) | 705-546-147 | 1:250 |
| Cy3 (donkey, anti-rat) | Jackson ImmunoResearch | 712-296-150 | 1:1,000 |
| Horseradish peroxidase (goat, anti-rabbit) | Jackson ImmunoResearch | 111-055-003 | 1:500 |
CA-II, carbonic anhydrase 2 (gene: Car2); Lcn2/Ngal, lipocalin 2/neutrophil gelatinase-associated lipocalin.