Fig. 10.
The stimulatory effect of ANG II on ROMK channel activity in HK-fed rats is mediated by cGMP-related PDEs. A: representative traces of the stimulatory effect of cilostamide (10 μM), a specific inhibitor of PDE3, and ANG II (100 nM) on apical ROMK channel activity in a CCD from a HK-fed rat. See Fig. 1 for details describing the tracings. The experiment was performed in a cell-attached patch at a holding potential of 0 mV. B: cilostamide increased channel NPo but prevented further stimulation of NPo by ANG II.