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Localization of P2X4 receptors in A6 cells. Cell surface biotinylation of either the apical or basolateral side of A6 cells showed selective localization of P2X4 channels to the basolateral but not apical membrane (left). The blots on the right show that an apical protein [γ-epithelial Na+ channel (ENaC)] can be detected after apical biotinylation and that a basolateral protein (Na+-K+-ATPase) can be detected after basolateral biotinylation.
