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Effect of Ca2+ chelation with EGTA on P2X4 receptor-induced stimulation of ENaC. EGTA (2 mM) was applied apically 20 min before the addition of meSATP basolaterally. A: representative recording of ENaC current (∼25 min) after treatment with 2 mM EGTA (expanded section 1: ∼60 s) and after the addition of meSATP (expanded section 2: ∼60 s). B: effect of EGTA on ENaC Po before and after meSATP in 7 patches.
