Fig. 5.

Effect of acute in vivo treatment with AG-490 and β-lapachone. A and B: cumulative urinary volume (A) and time course of change in urinary osmolality (B) in rats housed in metabolic cages. Brattleboro rats were injected with AG-490 (35 mg/kg), β-lapachone (17 mg/kg), or vehicle intraperitoneally. Urine was collected every 2 h. Data are shown as means ± SE; n = 3. *P < 0.05 vs. vehicle. The experiment was performed twice, and the average of the two experiments was used for each rat. AG-490 treatment caused a highly significant reduction in urine volume and an increase in urine osmolality 2 and 4 h after injection.