Fig. 1.

Decreasing bacterial translocation attenuated hepatosteatosis 24 h after intoxication and burn injury. A: tight junction protein localization was assessed with antibody staining against zonula occludens protein-1 (ZO-1; green), phalloidin (red), and nuclei (blue) after treatment with saline vehicle, myosin light chain kinase (MLCK) inhibition with membrane-permeant inhibitor of kinase (PIK), or prophylactic antibiotics (Abx). Representative villi are shown at ×400 magnification. B: bacterial translocation to mesenteric lymph nodes after intoxication and burn injury reported as number of colony-forming units (CFU). C: liver sections stained with Oil Red O to visualize triglyceride accumulation at ×400 magnification. D: triglycerides in liver homogenates after intoxication and burn injury. *P < 0.05 vs. PIK and Abx (by Student's t-test). Values are means ± SE; n = 4–6 animals per group. Dashed line, sham-vehicle; dotted line, sham-ethanol; dashed-dotted line, burn-vehicle.