Fig. 6.

Measurement of parameters of Ca²⁺ handling in the heart. A and B: protein expression of sarco(endo)plasmic reticulum Ca²⁺-ATPase 2a (SERCA2; A) and phospholamban (PLB; B) was determined by Western blot analysis in hearts from control and diabetic (STZ) WT and 3M mice. WT and 3M samples were run on separate blots, with a common sample on all blots to allow for interblot comparison. Intensity of protein bands was quantified by densitometry, normalized to β-actin expression, and plotted as the fold change compared with the control WT sample. C: the ratio of PLB to SERCA2 was plotted separately. D: expression of ryanodine receptor 2 (Ryr2) was measured by real-time PCR and normalized to 18S mRNA expression. Values are expressed as fold changes compared with the control WT sample ± SE; n = 4–8. *P < 0.05 and +P ≤ 0.1 vs. respective control mice; #P < 0.05 vs. control WT mice.