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. Author manuscript; available in PMC: 2015 Sep 22.
Published in final edited form as: Chembiochem. 2014 Aug 14;15(14):2125–2131. doi: 10.1002/cbic.201402258

Figure 1.

Figure 1

p97 retains biochemical function when displayed on resin. a) Schematic representation of the loading of p97 on resin. b) ATPase analysis indicates that Affi-Gel 15 resin bearing p97 at a final solution concentration of 1 µM hexamer displays comparable activity as 1 µM p97 hexamer in 20 mM HEPES, 150 mM KCl, 25 mM MgCl2, 2 mM BME, 5% glycerol, 5 mM ATP, pH 7.4. c) Co-factor-binding is maintained as demonstrated by the reduction in p97’s ATPase activity upon adding 3.3 µM p47 monomer to resin bearing 1 µM p97 hexamer. d) p97-resin can specifically capture p47 from a cell extract. The five lanes are given as follows: Ly – crude lysate, w1–w3 – three independent washes, and E – denaturing elution. Samples were separated by 12% SDS-PAGE and visualized with a phosphorimager.