Table 3.
Literature overview of fermentation studies on C. saccharolyticus grown on pure sugar substrates. * Yields in mol H2/mol hexose; nd, not determined; ** For batch cultivations the maximal productivity is given; nd, not determined; B, batch cultivation; and CB, controlled batch cultivation; Chem, chemostat cultivations.
| Reference | Substrate | Substrate | H2 Yield * | Productivity ** | Cultivation method | Dilution rate (h−1) | Remarks |
|---|---|---|---|---|---|---|---|
| load (g/L) | (mmol/(L*h)) | ||||||
| [100] | Glucose | 10 | 3.0 | 20.0 mol/(g*h) | CB | ||
| 10 | 3.4 | 23.6 mol/(g*h) | CB | No YE in medium | |||
| 4 | 3.5 | 10.1 mol/(g*h) | Chem | 0.05 | |||
| 4 | 3.5 | 10.4 mol/(g*h) | Chem | 0.05 | No YE in medium | ||
| [75] | Glucose | 5 | 3.5 | 5.2 | Chem | 0.05 | |
| 5 | 2.9 | 11.0 | Chem | 0.15 | Residual glucose (3 mM) | ||
| 5 | 1.8 | 2.5 | Chem | 0.05 | no sparging, open gas outlet | ||
| 5 | wash out | wash out | Chem | 0.15 | no sparging, open gas outlet | ||
| [101] | Glucose | 5 | nd | nd | B | Extracellular proteome | |
| [91] | Glucose | 10 | 3.4 | 12.0 | CB | ||
| 31 | 2.8 | 12.9 | CB | Residual glucose | |||
| [93] | Sucrose | 10 | 2.9 | 7.1 | CB | ||
| [94] | Glucose/Xylose/Sucrose | 10 | 3.2 | 10.7 | CB | Sugar mix | |
| (6:2.5:1.5, w/w/w) | 20 | 2.8 | 9.4 | CB | Sugar mix | ||
| [55] | Glucose | 10 | 3.2 | 11.2 | CB | ||
| 20 | 3.4 | 12.2 | CB | ||||
| Fructose | 10 | 2.6 | 13.2 | CB | |||
| 20 | 2.4 | 13.4 | CB | ||||
| Glucose/Fructose | 10 | 3.0 | 13.2 | CB | Sugar mix | ||
| (7:3, w/w) | 20 | 2.6 | 12.2 | CB | Sugar mix | ||
| [35] | Xylose | 5 | nd | nd | B | Proteome data | |
| Glucose | 5 | nd | nd | B | Proteome data | ||
| [50] | Glucose | 5 | nd | nd | B | Extracellular proteome | |
| [79] | Sucrose | 4 | 2.7 | 23.0 | CB | ||
| 4 | 3.1 | 11.8 | CB | CO2 sparging | |||
| Glucose | 4 | 3.0 | 20.0 | CB | |||
| 4 | 2.7 | 12.0 | CB | CO2 sparging | |||
| [53] | Glucose | 0.5 | nd | nd | B | Microarray data | |
| Mannose | 0.5 | nd | nd | B | Microarray data | ||
| Arabinose | 0.5 | nd | nd | B | Microarray data | ||
| Xylose | 0.5 | nd | nd | B | Microarray data | ||
| Fructose | 0.5 | nd | nd | B | Microarray data | ||
| Galactose | 0.5 | nd | nd | B | Microarray data | ||
| mix (0.5 g/L each) | 3 | nd | nd | B | Microarray data, Sugar mix | ||
| [54] | Glucose/Xylose | 10 | 3.4 | 12.0 | CB | Sugar mix | |
| (7:3 w/w) | 14 | 3.3 | 10.1 | CB | Sugar mix | ||
| 28 | 2.4 | 9.7 | CB | Sugar mix | |||
| [102] | Sucrose | 10.3 | 2.8 | 22.0 | Trickle bed | 0.2–0.3 | 400 L, non-axenic fermentation |
| [33] | Glucose | 4 | nd | nd | CB | Microarray data | |
| Xylose | 4 | nd | nd | CB | Microarray data | ||
| Rhamnose | 4 | nd | nd | CB | Microarray data | ||
| Glucose/Xylose (1:1, w/w) | 4 | nd | nd | CB | Microarray data | ||
| [56] | Glucose | 4.4 | 3.3 | 4.2 | Chem | 0.05 | |
| 4.4 | 3.6 | 8.9 | Chem | 0.10 | |||
| 4.4 | 2.9 | 9.5 | Chem | 0.15 | Residual glucose (3.3 mM) | ||
| 4.4 | 2.9 | 9.1 | Chem | 0.20 | Residual glucose (8.9 mM) | ||
| 4.4 | 3.1 | 11.0 | Chem | 0.30 | Residual glucose (12.4 mM) | ||
| 4.4 | 3.0 | 12.4 | Chem | 0.35 | Residual glucose (12.7 mM) | ||
| 1.9 | 4.0 | 4.0 | Chem | 0.09 | |||
| 1.9 | 3.3 | 9.9 | Chem | 0.30 | Residual glucose (0.6 mM) | ||
| 4.1 | 3.5 | 7.7 | Chem | 0.09 | |||
| 4.1 | 3.1 | 11.6 | Chem | 0.30 | Residual glucose (11.9 mM) | ||
| [78] | Glucose | 10 | 2.5 | 10.7 | CB | ||
| Xylose | 10 | 2.7 | 11.3 | CB | |||
| Glucose/Xylose (11:3, w/w) | 8.4 | 2.4 | 9.2 | CB | Sugar mix | ||
| [103] | Sucrose | 10 | 3.3 | 8.4 | CB |