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. 2014 Aug 25;105(8):083123. doi: 10.1063/1.4893878

FIG. 3.

FIG. 3.

Under an AC E-field (700 kHz, 20 V), Cy5-biotin molecules can be efficiently focused on the tips of nanowires in suspension, co-localized with QDs coated with streptavidin for enhanced biodetection. (a) Fluorescent images of QDs on an array of assembled Au nanowires taken through a band pass filter of 605 nm (585 nm to 625 nm). (b) Fluorescent images of Cy5-biotin molecules on the same arrays of Au nanowires taken through a 650 nm long pass filter. Fluorescent spectra taken on the nanowire tips when the AC E-field is (c) on and (d) off, demonstrating the effective E-field focusing and specific conjugation of QD and Cy5-biotin. The insets show the corresponding fluorescent images with pseudocolors. The scale bar is 5 μm. (e) The signals of QD and Cy-5 biotin with/without the E-field.