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A: expression of histone deacetylases (HDAC1) in both the nuclear (lanes 1 and 2) and cytosolic fractions (lanes 3 and 4) of bovine aortic endothelial cells (BAECs). B: HDAC1 and nitric oxide synthase 3 (NOS3) interact basally in BAECs shown by anti-NOS3 immunoprecipitation (IP) with immunoblot (IB) for HDAC1 from total BAEC lysate, N = 3. C: NOS3 and HDAC1 interact in COS7 cell-reconstituted system with overexpressed NOS3 and/or HDAC1-Flag tag. L, molecular mass ladder.
