Fig. 4.

HDAC1 small-interfering RNA (siRNA) knockdown in BAECs resulted in a 50% reduction in HDAC1 protein expression (A) compared with scramble siRNA control BAECs (B). C: HDAC1 knockdown resulted in an increase in nitrite production under basal and ET-1 (100 nM, 1 h)-stimulated states, whereas ionomycin (3 μM, 1 h)-stimulated nitrite production was not significantly affected by HDAC1 knockdown. D: NOS3 acetylation was similar between scramble and HDAC1 siRNA cells as quantified in E. F: NOS3 phosphorylation levels were not significantly different from scramble controls. G: densitometry of the phosphorylation sites of NOS3. N = 3–5. *P < 0.05 compared with scramble + vehicle; †P < 0.05 compared with respective vehicle treatment.