Fig. 1.

Urocortin 2 (Ucn2) elicits a time-dependent increase in phosphorylation of Akt, endothelial nitric oxide synthase (eNOS), and ERK1/2. Original immunoblots (top) and average data (bottom) of Ucn2-induced changes in phosphorylation of Akt at Ser473 (A; n = 11) and at Thr308 (B; n = 8), of eNOS at Ser1177 (C; n = 8), and of ERK1/2 at Thr202/Tyr204 (D; n = 9). Phosphorylation of Akt, eNOS, and ERK increased in a time-dependent manner. Average data of the ratio of phosphorylated to total Akt, eNOS, and ERK1/2 were normalized to the untreated control (Ctrl). *P < 0.05 and **P < 0.01 vs. Ctrl.