Fig. 4.

Effects of wortmannin, LY294002 and H89 on Ucn2-induced phosphorylation of Akt and eNOS. Original immunoblots (top) and average data (bottom) of Ucn2 (100 nM)-induced changes in phosphorylation of Akt at Ser473 (A; n = 9; and B; n = 16) and at Thr308 (C; n = 15) and of eNOS at Ser1177 (D; n = 14) in the absence and presence of wortmannin (0.3 μM), LY294002 (LY; 10 μM), and H-89 (5 μM). Average data of the ratio of phosphorylated to total Akt or eNOS were normalized to the untreated Ctrl. **P < 0.01 vs. Ctrl.