Fig. 9.

Scheme illustrating the signaling pathways activated by Ucn2 in rabbit ventricular myocytes to induce increased production of NO via eNOS phosphorylation at Ser1177. NO, in turn, stimulates sGC to increase cGMP concentration. Arrows indicate activation, phosphorylation or increased production. Enzyme inhibitors and receptor antagonists used in this study and their respective targets are shown with blunt arrows. The CRFR₂-PKA-dependent increase in [Ca²⁺]_i transients was demonstrated in a previous study in these cells (42). PDK, phosphoinositide-dependent kinase; FSK, forskolin; AC, adenylyl cyclase.