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. 2014 Apr 25;3(2):e000611. doi: 10.1161/JAHA.113.000611

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© 2014 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 3. — A, Quantitative analysis of peripheral blood leukocyte concentrations up to 21 days after anti‐CD4 antibody injection. Left panel: peripheral blood leukocytes, P=0.023 for the change in lymphocyte proportions between day 1 and 3 weeks (ANOVA); right panel: sub‐fractionation of lymphocytes into B cells and CD4⁺ T cells demonstrated the changing proportions from day 1 to 3 weeks (P=0.0021, ANOVA) and the pre‐post effect (P=0.006, paired t test). B, Left panel: kinetics of the LDPI perfusion ratio of pre‐immunized mice (normal saline vs CD4‐depletion vs CD4‐depletion plus ttMo transplantation, P<0.189, ANOVA); right panel: LDPI perfusion ratio 3 weeks after ligation of the femoral artery, the time point of maximal collateralization (P<0.001, t test). The transplantation of ttMo into non‐CD4⁺ T‐cell‐depleted mice is shown for comparison (black bar). ANOVA indicates analysis of variance; LDPI, laser Doppler perfusion imaging; lig, ligated; ttMo, tetanus toxoid monocytes; Unlig, unligated.