Skip to main content
. 2014 Apr 25;3(2):e000626. doi: 10.1161/JAHA.113.000626

Figure 14.

Figure 14.

Dynamics of NFAT5 expression/translocation and TNC expression in stretch‐stimulated HUASMCs. Cells were exposed to biomechanical stretch for 6, 24, and 48 hours (0.5 Hz, 0% to 13% elongation). Western blot analyses of nuclear protein fractions (H‐H3: histone H3) and cytoplasmic protein fractions (A) as well as detection of NFAT5‐specific immunofluorescence in the nuclei (B) reveal a robust nuclear localization of NFAT5 24 hours upon onset of stretch stimulation (A, *P<0.05 vs control; n.s.—not significant vs control; A, n=3 for all experimental groups (repeated measures ANOVA); B, n.s.—not significant vs control, *P<0.05 vs control with n=5 for control, n=3 for 6 hours, n=6 for 24 hours and n=3 for 48 hours). Tenascin‐C (TNC) mRNA expression is significantly increased in HUASMC exposed to stretch for 24 but not 6 hours (C, n.s.—not significant vs control, *P<0.05 vs control, with n=5 for control, n=3 for 6 hours, n=3 for 24 hours, and n=3 for 48 hours; TNC expression was normalized to RPL32 expression). HUASMCs indicates human arterial smooth muscle cells; RPL32, ribosomal protein L3.