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Lipocalin‐2 binds with linoleic acid with high affinity. Thermodynamic parameters of nonesterified free fatty acids (NEFAs) binding to hlipocalin‐2 were determined by isothermal titration microcalorimetry. Changes in Gibbs free energy (ΔG) and equilibrium dissociation constant (Kd) are presented as means±SEM. *P<0.01 versus other conditions; n=5 in all groups.
