Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 25;3(2):e000434. doi: 10.1161/JAHA.113.000434

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Figure 8. — MicroR‐23b (miR‐23b) downregulation is dependent on p38 MAPK pathway. A, p38 MAPK, ERK1/2, and JNK were significantly more phosphorylated in the myocardial samples from T2‐DM compared with NDM as shown by representative Western blots and O.D. semiquantitative analysis; *P<0.001 vs NDM, n=20 per group. B, High glucose (HG) significantly increased the phosphorylation levels of p38, ERK1/2, and JNK in nCMs in vitro. *P<0.001 vs normal glucose (NG), n=4 per group. C, HG significantly increased the phosphorylation levels of p38, ERK1/2, and JNK in arCMs in vitro. *P<0.0001 vs NG, n=4 per group. D, SB‐203580, PD‐98059, and SP‐600125 (p38, ERK1/2, and JNK specific inhibitors, respectively) effects on miR‐23b levels in high‐glucose (HG) treated nCMs or arCMs in vitro. *P<0.001 vs normal glucose (NG). E, SB‐203580, PD‐98059, and SP‐600125 effects on CA‐II mRNA levels in high‐glucose (HG)‐treated nCMs or arCMs in vitro. *P<0.03 vs normal glucose (NG), n=4 per group. F, SB‐203580 reduced HG‐induced nCM growth in vitro. *P<0.003 vs normal glucose (CON), n=4 per group. G, SB‐203580 reduced HG‐induced arCM apoptosis in vitro. *P<0.0008 vs normal glucose (CON), n=4 per group. Quantitative data are expressed as mean±SE. DM‐T2 indicates diabetes mellitus type 2; nCM, neonatal cardiomyocyte; arCM, adult ventricular cardiomyocyte; NDM, non–diabetes mellitus.