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. 2014 Jul 22;2(7):e12086. doi: 10.14814/phy2.12086

Figure 6.

Figure 6.

Characterization of the levels of mRNA encoding cubilin, amnionless and megalin in human fetal small intestine including terminal ileum. (A) RT‐PCR using RNA isolated from a human fetal terminal ileum biopsy (16–20 weeks gestation). Lane 1: GAPDH = 63 bp, lane 2: CUBN = 71 bp, lane 3: AMN = 116 bp, and lane 4: LRP2: 71 bp and primer dimers ~ 50 bp. (B) RT‐PCR using RNA isolated from a cell line generated from human fetal small intestine. Lane 1: CUBN = 153 bp, lane 2: AMN = 116 bp, lane 3: LRP2 =238 bp, lane 4: GAPDH = 63 bp. (C) qPCR: qPCR using cDNA generated from RNA isolated from a cell line generated from human fetal small intestine. (D) RT‐PCR using a commercially available total RNA pool purified from human fetal small intestine. Lane 1: CUBN = 153 bp, lane 2: AMN = 116 bp, lane 3: LRP2 =238 bp, lane 4: GAPDH = 63 bp. All PCR products were analyzed on a 2% agarose gel and visualized using SybrGreen.