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. 2014 Jul 17;2(7):e12075. doi: 10.14814/phy2.12075

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Low oxygen tension enhances the formation of EBs (embryoid bodies). Representative phase‐contrast images of EBs on day 3 and day 5 generated from (A) ESC and (B) iPSC, under 20% and 5% oxygen. Quantification of EBs generated from (C) ESC and (D) iPSC in suspension culture on day 3 and day 5 under 20% and 5% oxygen tensions. Percentage of EBs on day 3 generated from (E) ESC and (F) iPSC that adhered onto gelatin‐coated plates after 2 days in culture in the presence of 0.5% serum (low serum) or serum‐free supplemented medium and activin A under 20% and 5% oxygen tensions. (G) Representative phase‐contrast images of ESC‐ and iPSC‐derived cultures on day 5, after EB formation and adhesion onto gelatin‐coated plates for 2 days. Data collected from N =3 independent experiments. Statistical analysis was performed by Student's t‐test and P <0.05 (*) indicated significant difference between groups. ESC, embryonic stem cells; iPSC, induced pluripotent stem cells.