Comparison of the levels of RNA and proteins encoded by nuclear DNA and mtDNA. (Left) Northern blot analysis of RNA resulting from transcription of nuclear DNA (ACT1, NUBM, NUCM, NESM) and mtDNA (23S rRNA, ATP6). (Right) Western blot analysis of the levels of mitochondrial proteins encoded by nuclear DNA (NUCM [49-kDa], NUBM [51-kDa], and NESM subunits of complex I) and mtDNA (Atp6p and Cox2p). The WT (strain Po1h) and Δmhb1 protein extracts were prepared from the same numbers of cells. Note that whereas anti-NUCM, anti-NUMB, and anti-NESM antibodies were raised against Y. lipolytica proteins, anti-Atp6p and anti-Cox2p antibodies were raised against S. cerevisiae Atp6p and Cox2p, respectively.