FIG 3.

Amount of Efg1 in the nucleus of cells rapidly decreases upon shift to hypha-inducing conditions. WT cells containing an allele of EFG1 with a C-terminal MYC epitope tag was shifted to Spider medium at 37°C. Samples were harvested at the indicated time points and processed for immunofluorescence with anti-Myc primary and Texas red secondary antibodies. Nuclei were stained with DAPI as described in Materials and Methods.