FIG 2.

NTH1 and NTH2 are differentially expressed. (A) NTH2 is induced during sexual development. Lanes: 1 and 2, a and α yeast grown in rich medium (YPD liquid); 3, a and α yeast mixed under cross conditions (V8 agar, pH 7, at 22°C for 72 h); 4, spores from a cross after 72 h. Top, middle, and bottom images reflect probes to the transcripts of interest: NTH2, NTH1, and GPD1, respectively. (B) NTH2 is induced on V8 agar. Lanes: 1 and 2, a and α yeast grown on rich medium (YPD agar); 3 and 4, a and α yeast grown individually on V8 agar for 48 h; 5, a and α yeast grown in mixed culture on V8 agar for 48 h. Probes used were to NTH2 and GPD1. GPD1 was used as a loading and hybridization control.