TABLE 1.

Strains and oligonucleotides used in this study

Strain	Genotype	Reference/source or descriptiona
C. neoformans var. neoformans serotype D
JEC20	MATa	27
JEC21	MATα	27
CHY2083-2085	MATa nth2::NEOR	This study
CHY2089-2091	MATα nth2::NEOR	This study
CHY1705-1707	MATa nth1::NATR	This study
CHY1711-1713	MATα nth1::NATR	This study
CHY2182-2183, 2188	MATa nth1::NATR nth2::NEOR	This study
CHY2180-2181, 2184	MATα nth1::NATR nth2::NEOR	This study
Oligonucleotides used in cloning and PCR
CHO1565	TAATCAGTGGGTGAGGTGTG	NTH1 5′ Flank FWD
CHO1566	GCTCACATCCTCGCAGCCTCGAAGTTGGAGTGAAGGAGA	NTH1 5′ Flank REV
CHO1567	CTAGTTTCTACATCTCTTCCCTGGAAACATTCTTGATGG	NTH1 3′ Flank FWD
CHO1568	TCTCGCCCCTCCTAAACTAT	NTH1 3′ Flank REV
CHO1569	TCTCCTTCACTCCAACTTCGAGGCTGCGAGGATGTGAGC	NATR cassette FWD
CHO1570	CCATCAAGAATGTTTCCAGGGAAGAGATGTAGAAACTAG	NATR cassette REV
CHO2636	GTGTATCCAATCCAAGCACT	NTH2 5′ Flank FWD
CHO2637	GTCATAGCTGTTTCCTGGAGCTTTCGTTCATCAAGTC	NTH2 5′ Flank REV
CHO2764	CATCCTCGCAGCAAGGGCGTTTTGAGGTTCGAGGGTGAG	NTH2 3′ Flank FWD
CHO2765	CCCATACTACCGCAGCAATC	NTH2 3′ Flank REV
CHO2640	GACTTGATGAACGAAAGCTCCAGGAAACAGCTATGAC	NEOR cassette FWD
CHO2641	GGCTATGTTCAATGGGTATCCGCCCTTGCTGCGAGGATG	NEOR cassette REV
CHO2979	GCCTTCTCGAGGGGCTGGGA	NTH1 northern probe FWD
CHO2980	CGTGGGCTTCGCCAGTCGTT	NTH1 northern probe REV
CHO2938	GCCGCCCCATGCGTGTAAGT	NTH2 northern probe FWD
CHO2939	CTCACCACCTCCTCCAGCGG	NTH2 northern probe REV

^aFWD, forward; REV, reverse.