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. 2014 Oct;13(10):1337–1345. doi: 10.1128/EC.00088-14

FIG 6.

FIG 6

Transfection of PfHly III-GFP into P. falciparum. (A) Schematic of PfHly III-GFP integrated into Dd2 genome. 5′1 (Cg6 5′), 5′2 (pLN-PfHly III rev), 3′1 (pLN-PfHly III-GFP for), and 3′2 (attR rev) represent primer annealing sites. (B) Predicted genetic locus and PCR verification of pLN-PfHly III-GFP integration into the Dd2attB site. The 5′1 and 5′2 primers amplify a 350-bp product indicative of the attL site, and the 3′1 and 3′2 primers amplify a 680-bp product indicative of the attR site, overall indicating integration into the targeted genetic locus. (C) Lane 1, PCR 720-bp amplicon with p1 and p2 primers demonstrating the presence of GFP DNA in PfHly III-GFP-transfected parasites; lane 2, positive control; lane 3, negative control; lane M, marker. (D) PCR 780-bp amplicon with p3 and p2 primers showing presence of PfHly III-GFP DNA.