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Wild-type haploid cells or haploid cells with ump2 disruption (Δump2) or ump2 overexpression (ump2otef) were grown either on YEPS medium (yeast extract-peptone-dextrose; a carbon/nitrogen-replete medium) or on SLAD (synthetic low-ammonium dextrose) medium for 4 h. Haploid cells overexpressing ump2 showed filamentous growth, even in nitrogen- and carbon-replete medium. Wild-type cells (insets and right panels) were grown under the same conditions as the mutants.
