TABLE 1.
Strains used in this study
| Strain | Description | Genotype | Source |
|---|---|---|---|
| SC5314 | Wild type | 70 | |
| CAI4 | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 | 71 | |
| AFA60a | CAI4 transformed with URA3 vector | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 RPS10::CIp10 | 42 |
| AFA59b | CAI4 carrying extra copy of CaERG11 under the control of ACT1 promoter | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 RPS10::pAFC89b | 42 |
| DSY448 | cdr1Δ/cdr1Δ mutant | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 cdr1Δ::hisG-URA3-hisG/cdr1Δ::hisG | 72 |
| DSY465 | mdr1Δ/mdr1Δ mutant | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 benΔ::hisG-URA3-hisG/benΔ::hisG | 72 |
| CMDR1E2A and -B | CAI4 carrying CaMDR1 under the control of ADH1 promoter | ura3Δ::imm434/ura3Δ::imm434 iro1Δ/iro1Δ::imm343 ADH1/adh1::PADH1-MDR1-URA3 | 39 |
| SN152 | Background strain TF wild type and TF077 | arg4Δ/arg4Δ leu2Δ/leu2Δ his1Δ/his1Δ URA3/ura3Δ::imm434 IRO1/iro1Δ::imm343 | 73 |
| TF wild type | Control wild-type strain (paired “wild-type” TF077) | arg4Δ/arg4Δ leu2Δ/LEU2 his1Δ/HIS1 URA3/ura3Δ::imm434 IRO1/iro1Δ::imm343 | 31 |
| TF077 | upc2Δ/upc2Δ mutant | As SN152, but upc2Δ::HIS1/upc2Δ::LEU2 | 31 |
| BWP17 (TW14901) | Background strain D-6 and EC-7 | ura3Δ::imm434/ura3Δ::imm434 iro1/iro1Δ::imm434 his1Δ::hisG/his1Δ::hisG arg4Δ/arg4Δ | 22 |
| D-6 (TW14903) | upc2Δ/upc2Δ mutant | As BWP17 but upc2Δ::URA3/upc2Δ::ARG4 | 22 |
| EC-7 (TW14904) | Reconstituted strain | As D-6 but upc2Δ::URA3/upc2Δ::UPC2-HIS1 | 22 |
| pTET-ERG11 | CAI4 with doxycycline-repressible ERG11 as the only ERG11 allele | As CAI4, but tetO-ERG11/erg11Δ | 41 |
| CaLC1411 (CaLC436) | Strain with doxycycline-repressible HSP90 as the only HSP90 allele | arg4Δ/arg4Δ his1Δ/his1Δ URA3/ura3Δ::imm434 IRO1/iro1Δ::imm434 HIS1/his1Δ::TAR-FRT hsp90Δ::CdHIS1/his1Δ::TAR-FRT | 74 |
| SC2H3 | One- and two-hybrid reporter strain in the SN152 background | Like SN152, but 5 × LexAOp-ADH1b/HIS1 5 × LexAOp-ADH1b/lacZa | 27 |
| OX control | Strain carrying empty vector under MET3 promoter | As SC2H3, but MET3pro::lexA | Stynen et al., unpublished |
| Upc2 OX | Strain carrying extra copy of UPC2 under MET3 promoter | As SC2H3, but MET3pro::lexA::UPC2 | Stynen et al., unpublished |
| Gcn4 OX | Strain carrying extra copy of GCN4 under MET3 promoter | As SC2H3, but MET3pro::lexA::GCN4 | Stynen et al., unpublished |
| Cap1 OX | Strain carrying extra copy of CAP1 under MET3 promoter | As SC2H3, but MET3pro::lexA::CAP1 | Stynen et al., unpublished |
| Dot6 OX | Strain carrying extra copy of DOT6 under MET3 promoter | As SC2H3, but MET3pro::lexA:DOT6 | Stynen et al., unpublished |
| Rtg3 OX | Strain carrying extra copy of RTG3 under MET3 promoter | As SC2H3, but MET3pro::lexA::RTG3 | Stynen et al., unpublished |
a
This is SN152, but it contains two reporter genes (HIS1 and LacZ), and the expression of these reporter genes is controlled by a promoter element consisting of 5 times the LexA operator sequence and the ADH1 basic promoter sequence.