AFLP analysis of H. pylori
sabA poly(T) tract mutants. AFLP analysis was used to quantify variations in the sabA poly(T) tract containing amplicons from H. pylori J99 and poly(T) tract mutants. The amplicon generated using primers sabA IG Fwd and sabA IG Rev (see Table S1 in the supplemental material) is predicted to be 230 bp based on the annotated sequence of H. pylori strain J99, where the sabA poly(T) tract possesses 18 thymines (31). Shown are WT H. pylori J99 (A) and the sabA poly(T)18 control mutant (B), sabA poly(T)13 mutant (C), sabA poly(T)16 mutant (D), sabA poly(T)17 mutant (E), sabA poly(T)22 mutant (F), and sabA poly(T)23 mutant (G).