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. 2014 Oct;196(19):3366–3376. doi: 10.1128/JB.01832-14

FIG 1.

FIG 1

A LigD-LIG-independent NHEJ pathway participates in chromosomal repair. (A) M. smegmatis ligD-(K484A) (MGM 803) and M. smegmatis ligD-(ΔLIG) (MGM 805) were subjected to 600 and 1,200 Gy of IR after 2 days in late stationary phase in 7H9 minimal medium alongside wild-type and ΔligD (MGM 140) strains. The surviving cells were enumerated as 10-fold serial dilutions. Treatment was carried out on biologic triplicates, and each treatment was spotted in duplicate. (B) Fraction survival of wild-type (wt), ligD-(K484A), and ΔligD strains was plotted in log scale with the IR dose on the x axis. Statistical significance between strains is indicated by a vertical line, with “*” indicating P < 0.05. (C) The fraction survival of wild-type, ligD-(ΔLIG), ligD-(K484A), and ΔligD strains. (D) The relative frequency of homologous recombination (HR) and single-stranded annealing (SSA) in the chromosomal I-SceI assay for the indicated strains.