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Role of PolD1 and PolD2 in NHEJ. Late-stationary-phase IR sensitivity of wild-type, ΔligD, and ΔpolD1 (MGM 843) strains. (B to D) ligD-(D136A-D138A) (MGM 801), ligD-(D136A-D138A) ΔpolD1 ΔpolD2 (MGM 846), and Δku (MGM 154) strains (B), ligD-(K484A) (MGM 803), ligD-(K484A) ΔpolD1 (MGM 844h), and Δku (MGM 154) strains (C), or plasmid NHEJ assay with 5′-overhang and blunt-end plasmids (D). Statistical significance between strains is indicated, with “*” indicating P < 0.05.
