TABLE 3.
Condition | Activity of fusion in AE104 strain: |
|||||
---|---|---|---|---|---|---|
Wild type | ΔfurC | ΔfurCb | ΔfurCc | ΔfurCd | ΔfurCe | |
MM | 20 ± 3 | 251 ± 30 | 229 ± 14 | 220 ± 37 | 11 ± 1 | 24 ± 5 |
200 μM Zn2+ | 13 ± 1 | 150 ± 7 | 140 ± 18 | 120 ± 35 | 8 ± 2 | 11 ± 3 |
500 μM Zn2+ | 11 ± 2 | 134 ± 22 | 111 ± 20 | 111 ± 27 | 7 ± 3 | 12 ± 2 |
200 μM EDTA | 65 ± 10 | 259 ± 21 | 256 ± 20 | 261 ± 25 | 59 ± 15 | 94 ± 6 |
500 μM EDTA | 78 ± 8 | 257 ± 20 | 243 ± 38 | 269 ± 29 | 61 ± 16 | 98 ± 7 |
Three experiments were performed, and deviations are shown.
With plasmid pBBR1.
With plasmid pBBR::furC with an N-terminal FurC tag.
With plasmid pBBR::furC with a C-terminal FurC tag.
With plasmid pBBR::furC.