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Product identification of reactions with CysO-thiocarboxylate (CysO-SH) by electrospray mass spectrometry. (A) Mass spectrum of intact CysO-SH (9,573.5 Da). (B and C) Mass spectrum of reaction product formed by CysK2, showing intact CysO-SH mass (9,571.6 Da) (B) and of the covalent CysO-cysteine adduct (9659.0) produced by CysMMtb in the second half-reaction (C). Both enzymatic reactions were performed in the presence of 10 μg CysO-SH (106 μM) and 0.5 mM OPS.
