TABLE 1.
Cysteine synthase |
O-Phosphoserine sulfhydrylase |
O-Phosphoserine S-sulfocysteine synthase |
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---|---|---|---|---|---|---|---|---|---|---|
Km (μM) |
kcat (min−1) |
kcat/Km (103 M−1 min−1) |
Km (μM) |
kcat (min−1) |
kcat/Km (103 M−1 min−1) |
|||||
OPS | Na2S | OPS | Na2S | OPS | Na2S2O3 | OPS | Na2S2O3 | |||
CysK2 | 135 ± 39.4 | 374 ± 233.6 | 12.1 ± 1.44 | 0.089 | 0.032 | 1,085.5 ± 82.3 | 43.9 ± 7.2 | 54.8 ± 1.8 | 0.050 | 1.248 |
CysK2R243A | 1,085.4 ± 306.8 | NDa | 0.4 ± 0.075 | 0.0061 | 485.1 ± 96.0 | 213.7 ± 13.1 | 27.4 ± 1.6 | 0.056 | 0.128 |
ND, not determined.