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. 2014 Oct;196(19):3410–3420. doi: 10.1128/JB.01851-14

TABLE 1.

Kinetic parameters of M. tuberculosis CysK2

Cysteine synthase O-Phosphoserine sulfhydrylase
O-Phosphoserine S-sulfocysteine synthase
Km (μM)
kcat (min−1) kcat/Km (103 M−1 min−1)
Km (μM)
kcat (min−1) kcat/Km (103 M−1 min−1)
OPS Na2S OPS Na2S OPS Na2S2O3 OPS Na2S2O3
CysK2 135 ± 39.4 374 ± 233.6 12.1 ± 1.44 0.089 0.032 1,085.5 ± 82.3 43.9 ± 7.2 54.8 ± 1.8 0.050 1.248
CysK2R243A 1,085.4 ± 306.8 NDa 0.4 ± 0.075 0.0061 485.1 ± 96.0 213.7 ± 13.1 27.4 ± 1.6 0.056 0.128
a

ND, not determined.