TABLE 2.

Requirements for an industrially relevant chassis cell

Requirement for a chassis cell
Overall simplicity
Minimal no. of carbon sinks and other nonoptimal flux paths
Predictable metabolic and regulatory networks (more control over growth and production)
Simplified translation code
Reduced genetic drift and limited evolvability
Robust mechanisms for genome replication, cytokinesis, and coordination in between
Robust cell membrane and cell wall that confers resistance to shear stress in bioreactors
Efficient transcription, translation, and regulation for optimization of cellular fluxes to desired goals
Availability of predictive mathematical models that save expensive trial resources
Process-specific modules for implementation of different industrial solutions (particular for each process)
Other stress tolerance mechanisms, such as:
Product tolerance
High substrate tolerance
Tolerance to low O2 levels