FIG 1.
Location of the phage BPs PR promoter. (A) Map of a segment of the BPs genome (coordinates 26,790 to 30,150) showing the locations of the early lytic promoter PR and the promoter Prep that expresses the repressor gene (gene 33) and the integrase gene (gene 32). The putative expression patterns are included above. Note that attP is located within the repressor gene (25). The map was generated using the program Phamerator (48). (B) Sequence of the BPs PR promoter (coordinates 29,470 to 29,512). The +1 site for PR transcription initiation corresponds to the first base of the gene 34 initiation codon (25), and putative hexamers at the −10 and −35 regions are shown in blue type. The three bases to the left of the −10 hexamer (labeled E) correspond to the position where a 5′-TGN-extended −10 motif is located in some promoters. The putative OR operator is highlighted in yellow. The consensus sequences for the −10 and −35 regions of mycobacterial SigA promoters reported previously are shown (8, 9). (C) Promoter activities are shown as fluorescence units resulting from expression of an mCherry reporter gene in M. smegmatis using both extrachromosomal and integration-proficient vectors. Expression of the BCG hsp60 promoter and the BPs PR promoter is compared to that of a promoterless vector in both M. smegmatis mc2155 and a BPs lysogen.