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Impact of interhexamer spacer length on PR promoter activity. (A) Sequence of PR showing mutations altering the interhexameric spacing of PR. Spacing was increased to 19 bp by insertion of a T base between positions A−27 and G−28, reduced to 17 bp by removal of A−29, or reduced to 16 bp by removal of both A−29 and G−28. (B) Fluorescence activities of PR promoter spacer mutants in M. smegmatis mc2155 and in a BPs lysogen.
