FIG 4.

Activities of P_R promoter mutations in genomically integrated contexts. (A) Expression of mCherry by the BPs P_R promoter and mutant variants integrated into either M. smegmatis mc²155 or a BPs lysogen. Plasmids pLO73, pLO74, and pLO75 are integrating plasmids containing no promoter insert, the hsp60 promoter, and the wild-type P_R promoter, respectively. Other plasmids correspond to four series beginning with pLO76, pLO77, pLO78, or pLO92 and contain increasing numbers of mutations in P_R, as shown in panel B. (B) The positions of mutations in the P_R promoter mutants are shown.