FIG 5.
A calibrated series of promoters for M. smegmatis gene expression. (A) Fluorescence activities are shown in relative fluorescence units for a selection of promoters with increasing steps of activity in M. smegmatis mc2155 and in a BPs lysogen. Descriptions of the mutations in each plasmid are shown in Fig. 4. (B) Representative streaks of bacterial strains carrying the plasmids shown in panel A showing red fluorescence by mCherry. Notations below the photograph indicate the promoterless vector (vec), the hsp60 promoter (hsp60), and increasing mCherry fluorescence in PR derivatives.