TABLE 2.
Confirmed mutations in CuR1
| Genome coordinate | Substitution and/or description | Msed ORF | Protein | Mutation location | Domain affected |
|---|---|---|---|---|---|
| 155,137 | C→T, nonsynonymous (Glu→Lys) | 0191 | Ornithine cyclodeanimase | nt 691/939; aa 231/312 | NADB Rossman superfamily |
| 448,008 | C→A, nonsynonymous (Gly→Val) | 0490 | Heavy-metal translocating ATPase | nt 5/2235; aa 2/744 | Leader sequence |
| 500,688 | C→T, nonsynonymous (Glu→Lys) | 0545 | Hypothetical | nt 187/207; aa 63/68 | |
| 898,334 | C→T, nonsynonymous (Pro→Ser) | 0972 | Major facilitator transporter | nt 1009/1164; aa 337/387 | MFS superfamily (in region with putative substrate translocation pores) |
| 1,187,052 | 1-nt insertion of G; frameshift starting at aa 160 and premature stop after aa 199 | 1213 | Pyruvate dehydrogenase | nt 478/1035; aa 160/344 | TPP enzyme superfamily |
| 1,255,967 | C→T, nonsynonymous (Ser→Leu) | 1279 | Transcriptional regulator, MarR family | nt 344/453; aa 115/150 | HTH superfamily |
| 1,318,092 | 1-nt deletion; frameshift starting at aa73 | 1343 | Hypothetical (close BLAST hit to nucleotide binding domain-containing protein) | nt 217/243; aa 73/81 | Outside domains (close to C-terminal end of protein) |
| 1,314,441 | T→C, nonsynonymous (Leu→Pro) | 1338 | Hypothetical (close hit to bacterial regulatory protein, ArsR family) | nt 245/846; aa 82/281 | Outside domains |
| 1,377,170 | C→T, nonsynonymous (Tyr→Asn) | 1408 | Hypothetical | nt 175/309; aa 59/103 | |
| 1,377,775 | T→G, nonsynonymous (Thr→Pro) | 1409 | Hypothetical | nt 46/474, 16/157 | |
| 1,397,090 | T→G | 1427 | Pseudogene | nt 597/1217 | |
| 1,479,994 | T→C, nonsynonymous (Leu→Ser) | 1516 | Hypothetical (low-score BLAST hit to sodium-dependent phosphate transporter) | nt 806/975; aa 269/374 | No conserved domains in protein |
| 1,481,054 | 1-nt insertion of G; extends ORF by 627 nt | 1517 | Pseudogene | nt 170/854 | Extends ORF to include PHO4 superfamily |
| 1,783,624-1,783,625 | 2-nt insertion | 1838 | Pseudogene (upregulated in transcriptomic data but no coding sequence) | nt 631/2282 | Pseudogene |
| 1,783,847 | 1-nt insertion | 1838 | Pseudogene | nt 855/2282 | Pseudogene |
| 1,784,992 | T→C | 1838 | Pseudogene | nt 1838/2282 | Pseudogene |
| 1,827,819 | T→C, nonsynonymous (Leu→Ser) | 1884 | Glutamate synthase | nt 1512/2139; aa 504/712 | Outside domains |