FIG 1.

Colocalization of ε (YkzG) and RNAP. (A) Pseudocolored image of DAPI-stained exponentially growing B. subtilis EU164 (rpoY-gfp rpoC-mCherry) to label nucleoid location and morphology. (B) Pseudocolored image of the RpoC-mCherry fusion illustration of the subcellular location of RNAP. (C) Pseudocolored image of the ε (YkzG)-GFP fusion. (D) Overlay of the three fluorescence images shown in panels A to C. (E) Pseudocolored image of DAPI-stained nucleoids in B. subtilis BS491 (rpoC-gfp ΔrpoY). (F) Pseudocolored image of the RpoC-GFP fusion. (G) Overlay of images from panels E and F. Arrows indicate a transcription focus which represents the site of rRNA synthesis, and a cartoon of a cell containing two nucleoids is shown in panels D and G as a reference. Scale bar, 3 μm.