FIG 2.

Astrocytes are resistant to ER stress-induced cell death. (A) Primary astrocytes, NPCs, and microglia were treated with Thaps (0.03 to 3 μM) or Tunic (0.1 to 10 μM) for 24 h followed by assessment of cell viability by WST-1 assay. (B) Astrocytes were treated with Thaps or Tunic for 24 h followed by immunoblotting for cleaved caspase 3. Osmotic stress (OS; 500 mM sucrose for 4 h) was included as a positive control. Astrocytes were treated with Thaps (1 μM, 2 h) followed by immunoblotting (C), and CHOP mRNA was measured by qRT-PCR at the indicated times (D).