FIG 2.

EZH2 depletion is not associated with major cell cycle defects. (A) Cell cycle distribution analyses of HT1080 cells treated with siRNAs against EZH2 or luciferase for 72 h. siEZH2-A-treated cells displayed minor enrichment in G₂/M cells. (B) HT1080 cells were treated with siEZH2-A or with siLuci for 72 h, and cell extracts were analyzed by Western blotting to monitor the phosphorylation levels of H3 on Ser10 [pH 3(Ser10)]. Total H3 and β-actin levels are shown as loading controls, and EZH2 is shown as a control for siRNA knockdown efficiency.